Mattress model of lipid-protein interactions in membranes

Lipid-protein interactions in membranes.

The interactions of lipids with integral and peripheral proteins can be studied in reconstituted and natural membranes using spin label electron spin resonance (ESR) spectroscopy. The ESR spectra reveal a reduction in mobility of the spin-labelled lipid species, and in certain cases evidence is obtained for a partial penetration of the peripheral proteins into the membrane. The latter may be re...

Theoretical study of protein--lipid interactions in bilayer membranes.

An analysis is given for the perturbation of the order and composition of lipid bilayers near an intrinsic membrane protein. Two cases are examined: the protein influences the lipid order (i.e., "fluidity"), and the protein associates with one component of a lipid mixture preferentially. The order perturbation is studied as a function of temperature and lateral pressure by using Landau--de Genn...

Incorporation of outer membrane protein OmpG in lipid membranes: protein-lipid interactions and beta-barrel orientation.

OmpG is an intermediate size, monomeric, outer membrane protein from Escherichia coli, with n beta = 14 beta-strands. It has a large pore that is amenable to modification by protein engineering. The stoichiometry ( N b = 20) and selectivity ( K r = 0.7-1.2) of lipid-protein interaction with OmpG incorporated in dimyristoyl phosphatidylcholine bilayer membranes was determined with various 14-pos...

Lipid-protein interactions in biological membranes: a structural perspective.

Lipid molecules bound to membrane proteins are resolved in some high-resolution structures of membrane proteins. An analysis of these structures provides a framework within which to analyse the nature of lipid-protein interactions within membranes. Membrane proteins are surrounded by a shell or annulus of lipid molecules, equivalent to the solvent layer surrounding a water-soluble protein. The ...

Protein-lipid interactions on the surfaces of cell membranes.